Tested Studies in Laboratory Teaching, 2018, Volume 39
Alma E. Rodriguez Estrada
Abstract
Transformation of Escherichia coli with the Green Fluorescent Protein (GFP) is a laboratory
activity that has become increasingly popular at diverse levels ranging from middle school to
undergraduate courses. There are several mutant GFP genes that encode mutant proteins with
small differences in their nucleotide and amino acid sequences. This bioinformatics activity was
designed for an upper level course in molecular biology. Through this activity, students learn how
to use GenBank® (the Basic Local Alignment Search Tool, BLAST®) and the Protein Data Bank
(Benson et al. 2005, Berman et al. 2000) to analyze the gene and amino acid sequences of different
GFP variants while reviewing general concepts of gene and protein structure in addition to
primary literature.
Keywords: bioinformatics, GFP, green fluorescent protein, protein structure
University of Wisconsin, Madison (2017)
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